Dengue All test 1’s

Description

Dengue is a flavivirus, transmitted by Aedes aegypti and Aedes albopictus mosquitoes. It is widely distributed

throughout the tropical and subtropical areas of the world,1 and causes up to 100 million infections annually.2

Classic Dengue infection is characterized by a sudden onset of fever, intense headache, myalgia, arthralgia

and rash.

Primary Dengue infection causes IgM antibodies to increase to a detectable level in 3 to 5 days after the onset

of fever. IgM antibodies generally persist for 30 to 90 days.3 Most Dengue patients in endemic regions have

secondary infections,4 resulting in high levels of specific IgG antibodies prior to or simultaneous with IgM

response.5 Therefore, the detection of specific anti-Dengue IgM and IgG antibodies can also help to distinguish

between primary and secondary infections.

NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication.

NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form

a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to

be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or

secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown.

Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial

cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.

The Dengue IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid test that utilizes a combination of Dengue antigen coated colored particles for the detection

of IgG and IgM Dengue antibodies in human whole blood, serum, or plasma.

The Dengue NS1 Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid test that utilizes a combination of Dengue antibodies coated colored particles for the

detection of Dengue NS1 antigen in human whole blood, serum, or plasma.

How to use?

Dengue Combo Rapid Test Kits Characterized By A Sudden Onset Of Fever

PRINCIPLE

The Dengue IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) is a qualitative membrane-based immunoassay for the detection of Dengue antibodies in whole blood,

serum, or plasma. This test consists of two components, an IgG component and an IgM component. In the IgG

component, anti-human IgG is coated in IgG test line region. During testing, the specimen reacts with Dengue

antigen-coated particles in the test cassette. The mixture then migrates upward on the membrane chromatographically

by capillary action and reacts with the anti-human IgG in IgG test line region. If the specimen contains IgG

antibodies to Dengue, a colored line will appear in IgG test line region. In the IgM component, anti-human IgM

is coated in IgM test line region. During testing, the specimen reacts with anti-human IgM. Dengue IgM

antibodies, if present in the specimen, reacts with the anti-human IgM and the Dengue antigen-coated particles in the test cassette, and this complex is captured by the anti-human IgM, forming a colored line

in IgM test line region.

Therefore, if the specimen contains Dengue IgG antibodies, a colored line will appear in IgG test line region. If

the specimen contains Dengue IgM antibodies, a colored line will appear in IgM test line region. If the specimen

does not contain Dengue antibodies, no colored line will appear in either of the test line regions, indicating a

negative result. To serve as a procedural control, a colored line will always appear in the control line region,

indicating that the proper volume of specimen has been added and membrane wicking has occurred.

The Dengue NS1 Rapid Test Cassette (Whole Blood/Serum/Plasma) is a qualitative membrane-based immunoassay for the detection of Dengue NS1 antigen in whole

blood, serum, or plasma. During testing, the specimen reacts with Dengue antibody-conjugate in the test cassette. The Gold antibody conjugate will bind to Dengue antigen in the specimen sample

which in turn will bind with Anti-Dengue NS1 coated on the membrane. As the reagent moves across the

membrane, the Dengue NS1 antibody on the membrane will bind the antibody-antigen complex causing pale or dark pink line to form at the test line region of the test membrane. The intensity

of the lines will vary depending upon the amount of antigen present in the sample. The appearance of pink line

in the test region should be considered as positive result.

REAGENTS

The Dengue IgG/IgM Rapid Test Cassette contains Dengue antigen conjugated gold colloid particles,

anti-human IgM, anti-human IgG coated on the membrane.

The Dengue NS1 Rapid Test Cassette contains anti-Dengue Ag conjugated colloid particles, anti-Dengue Ag

coated on the membrane.

Applications:

The Dengue Combo Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of NS1 antigen and IgG and

IgM antibodies of Dengue virus in human whole blood, serum, or plasma as an aid in the diagnosis of Dengue

infections.

Specifications

Model

A rapid test for a qualitative test for the detection of NS1 antigen, IgG and IgM.

For medical and other professional in vitro diagnostic use only.

Format

Strip, Cassette

Accuracy

99.2%

Shelf life

24months

Component

Test cassettes, Droppers, Package insert, Sample dilution tube

Specimen

Serum/Plasma/Blood

Storage

2-30°C

Read time

20 mins

Others

The kits can be made according to the customers’ artwork or design

STORAGE AND STABILITY

Store as packaged at room temperature or refrigerated (2-30°C). The test is stable through the expiration date printed on the sealed pouch. The test must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date.